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KMID : 0895820170270010019
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Journal of Oriental Rehabilitation Medicine
2017 Volume.27 No. 1 p.19 ~ p.25
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Comparison of Index Compound Content and Anti-inflammatory Activity of Eucommiae Cortex by Various Drying Methods
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Do Ho-Jeong
Lee Jin-Ho
Ha In-Hyuk
Chung Hwa-Jin
Lee Jae-Woong
Kim Min-Jeong
Kim Eun-Ji
Lee In-Hee
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Abstract
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Objectives : Eucommiae cortex is one of the frequently used herbs for musculoskeletal disorders, with well-known anti-inflammatory effect. Meanwhile, powdered form of herbal drugs is more advantageous than decoction form, in that storing and taking is more convenient. Thus, the authors aimed to investigate whether the drying method affects the index compound level and anti-inflammatory effect of eucommiae cortex water extract.
Methods : Eucommiae cortex was extracted in distilled water at 100℃ for 3 hours, filtered, and then evaporated under vacuum. One half of the sample was freeze-dried at -80℃. Another half of the sample was added with dextrin and then spray-dried at 180℃. To assess the possible change in index compound content, pinoresinol diglucoside was selected as the index marker. The content level of the index compound in various extract sample was quantified through high performance liquid chromatography. In addition, iNOS assay in LPS-induced RAW 264.7 cells was adopted to determine the anti-inflammatory effect of various extract samples. Furthermore, MTT assay was performed to confirm that the result of iNOS assay was not due to cytotoxicity.
Results : There was no significance difference in index compound content between extract samples obtained through two different drying methods. Anti-inflammatory activity of extract samples were similar at the matching concentration, regardless of the drying methods. Extract samples did not show any significant cytotoxicity.
Conclusions : Extract samples of eucommiae cortex were obtained through freeze-drying and spray-drying. Neither change in index compound content nor difference in anti-inflammatory activity was observed between drying methods.
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KEYWORD
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Eucommiae cortex, Pinoresinol diglucoside, Freeze-drying, Spray-drying, Anti-inflammatory
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